复旦大学，生命科学学院，青年副研究员
随着高通量测序技术的飞速发展，外显子组和基因组测序鉴定出大量的肿瘤高频突变。根据肿瘤样本不同的突变谱特征，可将肿瘤进行分子病理分型。对高频突变基因在肿瘤发生发展过程中生物学意义的探索将为肿瘤的“精准治疗”提供重要的理论依据。我们的研究兴趣和研究方向主要是通过酵母双杂交、串联亲和纯化-质谱分析、siRNA文库和模式小鼠等技术手段，研究恶性肿瘤（如前列腺癌、肾癌和肝癌等）高频突变基因（特别是泛素化修饰相关基因）在肿瘤中的分子作用机制，阐明其生物学功能，并在大规模病理样本中验证，探索其成为肿瘤“精准治疗”分型依据和药物靶标的可能性。在该研究领域我们已建立起一整套较为完整的研究思路和技术平台，取得了一些重要的科研成果。

大学开始受教育经历
2004/09-2010/06    复旦大学   生命科学学院    医学遗传学专业    博士
2000/09-2004/06    四川大学   理科创新班       生物科学专业    本科

研究工作经历
2016/07-至今    复旦大学  遗传工程国家重点实验室   青年副研究员
2010/07-2016/06   复旦大学  遗传工程国家重点实验室   助理研究员

近年项目相关代表研究论文（*第一作者，§通讯作者）

1. Gao K*, Wang C*, Jin X, Xiao J, Zhang E, Yang X, Wang D, Huang H, Yu L, Zhang P. RNF12 promotes p53-dependent cell growth suppression and apoptosis by targeting MDM2 for destruction. Cancer Lett. 2016 May 28;375(1):133-41. 

2. Zhang Y, Wang Y, Wei Y, Wu J, Zhang P, Shen S, Saiyin H, Wumaier R, Wang C§, Yu L§. Molecular chaperone CCT3 supports proper mitotic progression and cell proliferation in hepatocellular carcinoma cells. Cancer Lett. 2016 Mar 1;372(1):101-9.   

3. Zhang P, Zhang Y, Gao K, Wang Y, Jin X, Wei Y, Peng J, Ma J, Wumaier R, Saiyin H, Tang Y, Wang D, Yu H, Dong Y, Huang H, Yu L§, Wang C§. ASPP1/2-PP1 complexes are required for chromosome segregation and kinetochore-microtubule attachments. Oncotarget. 2015 Dec 8;6(39):41550-65. 

4. Peng J, Ma J, Li G, Zhang P, Gao K, Jin X, Wang C§, Fan J§. Stabilization of MCRS1 by BAP1 prevents chromosome instability in renal cell carcinoma. Cancer Lett. 2015 Dec 1;369(1):167-74. 

5. An J, Ren S, Murphy SJ, Dalangood S, Chang C, Pang X, Cui Y, Wang L, Pan Y, Zhang X, Zhu Y, Wang C, Halling GC, Cheng L, Sukov WR, Karnes RJ, Vasmatzis G, Zhang Q, Zhang J, Cheville JC, Yan J, Sun Y, Huang H. Truncated ERG Oncoproteins from TMPRSS2-ERG Fusions Are Resistant to SPOP-Mediated Proteasome Degradation. Mol Cell. 2015 Sep 17;59(6):904-16.  

6. Gao K, Jin X, Tang Y, Ma J, Peng J, Wumaier R, Yu L, Zhang P§, Wang C§. Tumor suppressor SPOP mediates the proteasomal degradation of progesterone receptors (PRs) in breast cancer cells. Am J Cancer Res. 2015 Sep 15;5(10):3210-20.

7. Zhang P, Gao K, Jin X, Ma J, Peng J, Tang Y, Zhang Y, An J, Yan J, Dong Y, Huang H, Yu L§, Wang C§. Endometrial cancer-associated mutants of SPOP are defective in regulating estrogen receptor-α (ERα) protein turnover. Cell Death Dis. 2015 Mar 12;6:e1687.

8. Gao K, Tang W, Wang D, Zhang P, Yu L, Wang C§, Wu D§. Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils. J Cell Sci. 2015 Mar 1;128(5):992-1000.

9. Gao K, An J, Zhang Y, Jin X, Ma J, Peng J, Tang Y, Yu L, Zhang P§, Wang C§. The E3 ubiquitin ligase Itch and Yap1 have antagonistic roles in the regulation of ASPP2 protein stability. FEBS Lett. 2015 Jan 2;589(1):94-101. 

10. Zhang P, Gao K, Tang Y, Jin X, An J, Yu H, Wang H, Zhang Y, Wang D, Huang H, Yu L§, Wang C§. Destruction of DDIT3/CHOP protein by wild-type SPOP but not prostate cancer-associated mutants. Hum Mutat. 2014 Sep; 35(9):1142-51. 

11. An J*, Wang C*, Deng Y, Yu L, Huang H. Destruction of full-length androgen receptor by wild-type SPOP, but not prostate-cancer-associated mutants. Cell Rep. 2014 Feb 27;6(4):657-69.

12. Wang D, Zhang P, Gao K, Tang Y, Jin X, Zhang Y, Yi Q, Wang C§, Yu L§. PLK1 and β-TrCP-dependent ubiquitination and degradation of Rap1GAP controls cell proliferation. PLoS One. 2014 Oct 17; 9(10):e110296.

13. Yang Y*, Wang C*, Zhang P, Gao K, Wang D, Yu H, Zhang T, Jiang S, Hexige S, Hong Z, Yasui A, Liu JO, Huang H, Yu L. Polycomb group protein PHF1 regulates p53-dependent cell growth arrest and apoptosis. J Biol Chem. 2013 Jan 4;288(1):529-39.

14. Xiao Q*, Qu K*, Wang C*, Kong Y, Liu C, Jiang D, Saiyin H, Jia F, Ni C, Chen T, Zhang Y, Zhang P, Qin W, Sun Q, Wang H, Yi Q, Liu J, Huang H, Yu L. HDGF-related protein-3 is required for anchorage-independent survival and chemoresistance in hepatocellular carcinomas. Gut. 2013 Mar;62(3):440-51.

15. Wang C*, An J*, Zhang P, Xu C, Gao K, Wu D, Wang D, Yu H, Liu JO, Yu L. The Nedd4-like ubiquitin E3 ligases target angiomotin/p130 to ubiquitin-dependent degradation. Biochem J. 2012 Jun; 1;444(2):279-89.

16. Mao J*, Yu H*, Wang C*, Sun L, Jiang W, Zhang P, Xiao Q, Han D, Saiyin H, Zhu J, Chen T, Roberts LR, Huang H, Yu L. Metallothionein MT1M is a tumor suppressor of human hepatocellular carcinomas. Carcinogenesis. 2012 Dec;33(12):2568-77.

17. Liu G, Jiang S, Wang C, Jiang W, Liu Z, Liu C, Saiyin H, Yang X, Shen S, Jiang D, Zhou P, Han D, Hu X, Yi Q, Yu L. Zinc finger transcription factor 191, directly binding to β-catenin promoter, promotes cell proliferation of hepatocellular carcinoma. Hepatology. 2012 Jun;55(6):1830-9. 

18. Zhao J*, Wang C*, Wang J, Yang X, Diao N, Li Q, Wang W, Xian L, Fang Z, Yu L. E3 ubiquitin ligase Siah-1 facilitates poly-ubiquitylation and proteasomal degradation of the hepatitis B viral X protein. FEBS Lett. 2011 Oct 3; 585(19):2943-50.

19. Zhang P*, Wang C*, Gao K, Wang D, Mao J, An J, Xu C, Wu D, Yu H, Liu JO, Yu L. The ubiquitin ligase itch regulates apoptosis by targeting thioredoxin-interacting protein for ubiquitin-dependent degradation. J Biol Chem. 2010 Mar 19; 285(12):8869-79.